The functions of m-ant and m-pos on regulation of genes

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Part 1: The functions of m-ant and m-pos on regulation of genes
The mopholinos helps to bind the endogenous mRNA. They also give prevention against through steric interference. Their formation is through conversion of rib nucleosides to a ring structure of morpholine. They prevent the initiation of ribosome’s complex from binding to the cognate RNA. The m-ant and m-pos are highly soluble and stable. They are no-ionic and RNase-H independent. They also exhibit a high specifity.
The m-pos (morpholino phosphorodiamidate oligonucleotides) are soluble in water and quite resistant to a number of nucleases and proteases. These m-pos give an effective ribonucleic acid binding affinity thus a higher efficacy for anti-senses. The m-pos provide a sequence-specific inhibition on cell-transformation system with high concentration range. Upon hybridization of m-pos on the targeted RNA, it prevents translation initiation-pos change splicing depending on their designation whether to work against splice acceptor sites if designed to work against splice-donor sites. M-pos also have a therapeutic potential. The m-pos may also hinder the expression of genes in some organs such as the liver, the kidney and even the blood vessels.
The transparent embryos give a room for easy visualization of important process of development. When the acidity level drops within the endosome. The m-pos complex gets endocytosed leading to ionization of ethoxylated polythylenimine. This leads to endosomal membrane becoming more permeable thus enabling release of m-pos into the cytosol.
The m-ant (morpholino antisense oligos) helps to bind the pre-messenger or the messenger ribonucleic acid. This blocks access of the RNA. Compared to other antisense structural types, the morpholinos provide a better and efficient efficacy and an increased specifity. The morpholinos help to block nuclear processing, gene switching, act as anti-sense therapeuticals and also help in blocking the RNA translation.
Part 2
Ecdysteroids are typical hormones which diffuse into cells. They are meant to affect the expression of genes thus may cause an activation or deactivation base for certain types of genes. These may also result in formation or hindrance of formation of enzymes and other regulatory peptides. The alignment of the DNA genes which have been replicated form a banding pattern. The active gene transcription is represented by the visible puffs. The development stage of the insect is correlated with the patterns of the puffs.
The hormones induce early puffs and then they regress. This is followed by an increased number of early late and late puffs. Protein synthesis inhibitors do not affect the formation of puffs. These inhibitors prevent late puff induction. Hormone-receptor complex binding activates early puff genes thus representing formation of the late genes. Products of the early genes repress the activity of the early genes. The late genes function as the secondary gene responses helping inn morphogenesis. Transcription factors are encoded by these genes. Thus the steroid hormone is able to induce the expression of small sets of early genes which are regulatory. This overtime induces an increased set of genes which are used in the biological response to the hormones. The early genes are the zygotic while the late genes are the maternal. The products of the E-genes would be found at the middle of the cell. A zygotic gene gains its prenatal development at the earliest points. It is a changed version of a normal gene which is very crucial for the survival of the zygote.
REFERENCE
Mounton, J. (2007) Using Morpholinos to Control Gene Expression”. In Beaucage Serge, Current Protocols in Nucleic Acid Chemistry. New Jersey: John Wiley